Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Cytotoxic effect of 5-FU/Curcumin hybrids on SW620, HaCaT, and CHO-K1 cell lines.

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques:

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Data of viability (%) at the lowest and the highest concentrations evaluated.

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Concentration Assay

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: ( A ). Antiproliferative activity of hybrids 6a and 6d in SW480 cells; ( B ). Antiproliferative activity of hybrids 6d and 6e in SW620 cells. Data are presented as the mean ± SE of three independent experiments. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). Vehicle control was assumed to be 100% viable.

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Activity Assay, Control

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Cell cycle distribution after treatment with hybrids 6d and 6e in SW620 cells and representation of distribution percentages in bar chart form. All experiments were performed in duplicate and showed similar results. p -values lower than 0.05 were considered statistically significant (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques:

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Changes in ΔΨm and plasma membrane integrity. Treatment with hybrids 6a and 6d in SW480 and hybrids 6d and 6e in SW620 cells. PI+: PI-positive cells, with membrane damage or dead cells; DiOC6 High: live cells with high membrane polarization; DiOC6 Low: cells in latency that lose membrane polarization. Data are presented as the mean of these two populations ± SE of three independent experiments. p values lower than 0.05 were considered statistically significant (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Clinical Proteomics, Membrane

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Apoptosis of SW480 ( A ) and SW620 ( B ) cells following treatment with the hybrid compounds 6a , 6d , and 6e . The histograms represent one of at least two different experiments. Q1 + Q2: Cells that lost membrane integrity (late apoptotic cells, dead cells, necroptotic cells, secondary necrotic cells, others); Q3: Early apoptotic cells; Q4: Viable cells. Data are presented as the mean of these two populations ± SE of three independent experiments. p -values lower than 0.05 were considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Membrane

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Effect of 6d and 6e hybrids on apoptotic biomarker expression in SW620 cells. Results are the mean ± SE of two independent experiments. p -values less than 0.05 were considered statistically significant (* p < 0.05; ** p < 0.01).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Biomarker Discovery, Expressing

Journal: Oncology Letters

Article Title: A gut butyrate-producing bacterium Butyricicoccus pullicaecorum regulates short-chain fatty acid transporter and receptor to reduce the progression of 1,2-dimethylhydrazine-associated colorectal cancer

doi: 10.3892/ol.2020.12190

Figure Lengend Snippet: Effects of butyrate on CRC cells. (A) Effect of conditioned medium of B. pullicaecorum cultivation on CRC cell proliferation. Numbers of SW480 and SW620 cells were counted at days 1 and 5 following cell seeding. One-way ANOVA with Fisher's least significant difference (LSD) post-hoc test was performed to identify significant differences between groups. (B) Relative mRNA levels of SLC5A8. (C) Relative mRNA levels of GPR43. Gene quantitation was performed from SW480 cells and SW620 cells treated with 5 mM NaB for 48 h. Differences in gene expressions were compared using Student's t-test. Data are presented as mean ± SEM. *P<0.05. **P<0.01. CRC, colorectal cancer; NaB, sodium butyrate; SLC5A8, solute carrier family 5 member 8; GPR43, G-protein-coupled receptor 43; PY-X, peptone yeast extract broth; HRC, human reference cDNA.

Article Snippet: SW480 colon cancer cell line (ATCC CRL-1459; AJCC stage II) and SW620 lymph node metastatic derivative cell line (ATCC CRL-1831; AJCC stage III) from the same patient were purchased from American Type Culture Collection.

Techniques: Quantitation Assay